ABSTRACT
Objective To study the relationship between the abnormal blood fat components and the serum hypersensitivity C reaction protein concentration and the progression of diabetic retinopathy.Methods Fundus examination was carried out to inpatients in the Department of Endocrinology in the hospital from From July 2015to July 2017.These patients were divided into three groups according to results of fluorescein sodium contrast:The non retinopathy group (group A) , the non proliferative retinopathy group (group B) , the retinopathy proliferative phase group (group C) .The content of blood fat component and serum hypersensitivity C reaction protein in the experimental cases were measured, and the ratio of TC/HDL-C was calculated.The statistic software of SAS9.4was used for statistical processing.Results The concentration of the serum hypersensitive C reaction protein in patients with diabetic retinopathy was significantly increased and the ratio of TC/HDL-C increased significantly (P<0.05) .The hs-CRP concentration and TC/HDL-C ratio of the three study groups were compared with those of the healthy control group:the difference was statistically significant (P<0.05) .Among them, the hs-CRP concentration and TC/HDL-C ratio of group A were compared with group B and group C respectively and the difference were statistically significant (P<0.05) .There was no significant difference in hs-CRP concentration and TC/HDL-C ratio between group B and group C (P>0.05) .Linear correlation analysis was used to analyze the concentration of serum hypersensitive C reaction protein and the ratio of TC/HDL-C.It was found that there was a positive correlation between them, and there was a positive synergistic effect.Conclusion The measurement of serum hypersensitive C reactive protein has important clinical value for the discovery, evaluation and prognosis of diabetic retinopathy.The abnormalities of the blood fat component reflected by the TC/HDL ratio are also an important factor in assessing the progression of diabetic retinopathy.
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Objective To evaluate and select the dilution for high blood and urinary amylase among wet chemical & dry chemical de‐tection system ,hope to screen the optimum dilution for different measurement system and specimen type .Methods Pure water (H2 O) ,nor‐mal saline (NS) ,7% bovine serum albumin(7% BSA) ,low amylase specimen of serum and urine selected as candidate dilution .The high amylase specimen was diluted ,then was measured among wet chemical analyzer and dry chemical analyzer ,then the bias and correlation was evaluated according to the professional standard of National health and family planning commission of china .Results For high amylase ser‐um specimen ,the same dilution resulted in different bias between two detecting system .The bias of H2 O ,7% BSA and low amylase serum were less than the TEa (total error allowance) regulated by professional standard in wet chemical analyzer .Of them ,the low amylase serum was the best .In dry chemical analyzer ,the bias of H2 O ,NS and low amylase serum were no less than the professional standard .Of them ,NS was the best .For high amylase urine specimen ,the bias of H2 O and low amylase urine were less than the professional standard in wet chem‐ical analyzer ,and the low amylase urine was the best .In dry chemical analyzer ,only the bias of low amylase urine met the requirement of professional standard .The evaluation of linearity indicated that NS was unfit to the dilution for high amylase serum in wet chemical analyzer and for high amylase urine in dry chemical analyzer ,7% BSA was unsuitable to be the dilution for high amylase serum .Conclusion The effect of same dilution on different detecting system is different .The most suitable dilution is low amylase serum and urine for high amylase serum and urine specimen respectively in wet chemical analyzer ,and the suitable dilution is NS and low amylase urine respectively in dry chemical analyzer .
ABSTRACT
Objective To investigate the inhibitory effect of heparanase antisense oligodeoxynucleotide (AS-ODN) on the invasiveness of human liver cancer cell lines SMMC-7721,BEL-7402 in vitro. Methods We designed and synthesized AS-ODN that was complementary to the start codon region of heparanase mRNA,and the control,nonsense oligodeoxynucleotide (NS-ODN). The ODNs with the final concentration of 300 nmol/L was delivered into SMMC-7721,BEL-7402 cells by Oligofectamine TM Reagent. We evaluated heparanase gene expression using RT-PCR and detected heparanase protein expression using Western blot assay after transfection. Cell invasiveness was measured by Matrigel invasion assay.Results The result showed that heparanase gene expression,protein expression and invasiveness in AS-ODN group decreased significantly as compared with control groups( P